Phyllosticta neopyrolae Wikee, Motohashi, Crous & K.D. Hyde, Stud in Mycol 76: 20. 2013
Index Fungorum: IF803676; Facesoffungi: FoFxxxxx; MycoBank: MB803676.
Pathogenic on Pyrola asarifolia. Leaf spots orbicular to ellipsoid, black. Sexual morph: Unknown. Asexual morph: Conidiomata (on PNA) 60–100 × 60–113 μm, pycnidial, epiphyllous, sparse, solitary or aggregated, immersed at first, then erumpent breaking through the epidermis, brown to dark brown, subglobose. Peridium composed of the depressed or irregular cells in 1–4 layers, brown to dark brown, hyaline or paler toward the inside. Ostiole up to 10 μm diam., central. Conidiophores 15–20 × 2–3 μm, subcylindrical, reduced to conidiogenous cells, or with 1–2 supporting cells, branched at the base. Conidiogenous cells 8–15 × 2–3 μm, terminal, subcylindrical, hyaline, smooth, coated in a mucoid layer, proliferating several times percurrently near apex. Conidia (6–)7(–8) × (5–)6(–7) μm, solitary, hyaline, aseptate, thin and smooth walled, granular, or with a single large central guttule, broadly ellipsoid to globose, mucoid sheath and appendage lacking.
Culture characteristics: Colonies erumpent, spreading, with sparse aerial mycelium and feathery margins, covering the dish in 1 month. On MEA surface olivaceous-grey, reverse iron-grey; on OA irongrey; on PDA iron-grey on surface and reverse.
Representative isolates: Japan, Nagano, Sugadaira, on living leaf of Pyrola asarifolia subsp. incarnata, 17 June 2006, T. Hosoya, holotype TFM : FPH 7887, isotype CBS H-21395, MUCC 125 = CPC 21879 = CBS 134750 (ex-type culture).
Host: Pyrola asarifolia (Wikee et al. 2013).
Known distribution: Japan (Wikee et al. 2013).
GenBank Numbers: ITS - AB454318, LSU - AB454318, ACT - AB704233.
Notes: Two species of Phyllosticta are known from Pyrola spp., namely P. pyrolae Ellis & Everh. and P. pyrolae (Ehrenb. : Fr) Allesch. Of these, the latter species is an illegitimate homonym, with morphological characteristics (conidia 3–4 μm long) that indicate that it should be excluded from Phyllosticta s. str. (van der Aa & Vanev 2002). The other species, P. pyrolae Ellis & Everh. (conidia ovoid to globose, 4.5–7.5 × 4–9 μm, with mucoid layer and an apical appendage) resembles P. neopyrolae. Phyllosticta neopyrolae differs from these two species by having conidia that lack a mucoid sheath and apical appendage.
Aa HA van der, Vanev S (2002) A revision of the species described in Phyllosticta. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EHC, Hyde KD, Crous PW (2013) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Studies in Mycology 76: 1–29.