Phyllosticta mangiferae-indicae Wikee, Crous & K.D. Hyde, Stud in Mycol 76: 18. 2013
Index Fungorum: IF810593; Facesoffungi: FoFxxxxx; MycoBank: MB810593.
Endophytic on leaf of Mangifera indica. Sexual morph: Unknown. Asexual morph: Conidiomata pycnidial (on PNA), 220–300 × 160–180 μm, initially forming after 4 d of incubation, black, superficial, subglobose or ellipsoidal. Peridium composed of 1–3 layers of brown textura angularis, 20–30 μm thick. Conidiogenous cells 3–5 × 3–4 μm, lining the inner wall, phialidic, cylindrical, hyaline. Conidia (6–)9(–13) × (4–)5(–6) μm, ellipsoidal, hyaline, aseptate, smooth, surrounded by mucilaginous sheath, 0.5–2 μm thick, bearing single apical appendage, 3–14 μm long.
Culture characteristics: On OA colonies appeared flat, with irregular margins, initially hyaline with abundant mycelium, gradually becoming greenish after 2–3 d. On MEA, colonies woolly, irregular, initially white with abundant mycelium, gradually becoming greenish to dark green after 2–3 d, with white hyphae at the undulate margin, eventually turning black; reverse dark green to black. After 25 d in the dark at 27 ºC colony covering the whole plate. On PDA colonies flat, rather fast growing, initially white with abundant mycelium, gradually becoming greenish to dark green after 2–3 d with white hyphae at the margin, eventually turning black; reverse black and after 14 d in the dark at 27 ºC colony covering the whole plate.
Representative isolate: Thailand, Chiang Rai, Nanglae, on healthy leaf of Mangifera indica, July 2011, S. Wikee, holotype MFU13-0108, CPC 20274 = MFLUCC10-0029 = CBS 136061 (ex-type culture).
Host: Mangifera indica (Wikee et al. 2013).
Known distribution: Thailand (Wikee et al. 2013).
GenBank Numbers: ITS - KF170305, LSU - KF206240, TEF - KF289190, ACT - KF289296, GPDH - KF289121.
Notes: Phyllosticta mangiferae-indicae was isolated as an endophyte from a healthy leaf of Mangifera indica. Several species have been reported as pathogens on M. indica including G. mangiferae and P. brazilianiae (Glienke et al. 2011). Phyllosticta mangiferae-indicae produce abundant conidia on OA and form appressoria within 2 d. Morphologically, it is distinct from P. capitalensis (conidia 8–11 × 5–6 μm) in having longer conidia (conidia 6–13 × 4–6), and represents a distinct lineage with 99 % bootstrap support with the inclusion of TEF and GPDH sequence data. It is phylogenetically distinct from P. mangiferae, and most closely related to P. brazilianiae, which occurs on the same host.
Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EHC, Hyde KD, Crous PW (2013) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Studies in Mycology 76: 1–29.