Phyllosticta leucothoicola Wikee, Motohashi & Crous, Stud in Mycol 76: 18. 2013
Index Fungorum: IF805657; Facesoffungi: FoFxxxxx; MycoBank: MB805657.
Pathogenic on leaf pf Leucothoe catesbaei. Leaf spots purple-brown, scattered, enlarged and becoming confluent, subcircular to oblong, with brown to dark brown border. Sexual morph: Unknown. Asexual morph: Conidiomata (on PNA) up to 300 μm diam., pycnidial, mostly aggregated in clusters, black, erumpent, globose to clavate or elongated with necks up to 500 μm long, exuding colourless to opaque conidial masses. Peridium up to 40 μm thick, composed of several layers of textura angularis, inner wall of hyaline textura angularis. Ostiole up to 15 μm diam., central. Conidiophores 6–20 × 3–4 μm, subcylindrical to ampulliform, reduced to conidiogenous cells, or with 1 supporting cell. Conidiogenous cells 6–15 × 3–4 μm, terminal, subcylindrical, hyaline, smooth, coated in a mucoid layer, proliferating several times percurrently near apex. Conidia (6–)7–8(–9) × 6(–7) μm, solitary, hyaline, aseptate, thin and smooth walled, coarsely guttulate, or with a single large central guttule, ovoid to irregularly ellipsoid, at times enclosed in a thin mucoid sheath, up to 1.5 μm thick; apical mucoid appendage not seen. Spermatia developing in same conidioma as conidia, bacilliform, smooth, hyaline, guttulate, 5–7 × 2–3 μm.
Culture characteristics: Colonies erumpent, spreading, with sparse aerial mycelium and feathery margins, covering the dish in 1 month. On MEA surface olivaceous-grey, reverse iron-grey; on OA iron-grey; on PDA iron-grey on surface and reverse.
Representative isolate: Japan, Tokyo, on living leaf of Leucothoe catesbaei, May 1996, J. Takeuchi, holotype CBS H-21394, MUCC 553 = CPC 21881 = CBS 136073 (ex-type culture).
Host: Leucothoe catesbaei (Wikee et al. 2013).
Known distribution: Japan (Wikee et al. 2013).
GenBank Numbers: ITS - AB454370, LSU - AB454370, ACT - KF289310.
Notes: Phyllosticta leucothoës has been described from Leucothoe acuminata, although van der Aa & Vanev (2002) transferred this to Fusicoccum based on an examination of type material. Phyllosticta leucothoicola represents a distinct taxon on L. catesbaei, corroborating the morphological differences noted by Motohashi et al. (2009).
Aa HA van der, Vanev S (2002) A revision of the species described in Phyllosticta. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
Motohashi K, Inaba S, Anzai K, Takamatsu S, Nakashima C (2009) Phylogenetic analyses of Japanese species of Phyllosticta sensu stricto. Mycoscience 50: 291–302.
Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EHC, Hyde KD, Crous PW (2013) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Studies in Mycology 76: 1–29.